This study features LKC’s UTAS BigShot Ganzfeld System SG-2002 to evaluate the role of AQP4 in the mouse retina. Mice electroretinograms were recorded highlighting the quality and accuracy of ERGs obtained with LKC’s products.

The bidirectionalwater channel aquaporin 4 (AQP4), the main water channel protein, is abundantly expressed in the neural tissue. It regulates extracellular water space, waste clearance, calcium signaling and potassium buffering. The advantages and disadvantages of AQP4 neural tissue deficiency under pathological conditions, such as inflammation, and relationship with neural diseases, such as Alzheimer’s disease, have been previously reported. However, the physiological functions of AQP4 are not fully understood. The role of AQP4 in the mouse retina using Aqp4 knockout (KO) mice is evaluated in this paper.

Method

  • C57/B6J WT and KO for AQP4 mice
  • Immunohistochemistry for AQP4 localization in the retina
  • ERG recordings: scotopic and photopic electroretinograms using PowerLab System and LKC Technologies Ganzfeld System SG-2002 as stimulator
  • Total retina RNA
  • Primary Muller cell culture
  • Intravitreal KCl injections